Fig 1: Effect of PROKR antagonists on the permeability of the BBB co-culture model. Measurement of the permeability of the co-culture model was assessed in the presence or absence of PROK1 (30 nM) (A) or PROK2 (60 nM) (B), pretreated with PROKR antagonists, PC7 or PKR-A (1 µM). Data are presented as a percentage of permeability coefficient (Pe) compared to untreated insert, as mean ± SEM, from three independent experiments. Statistical analysis: unpaired two-tailed t-test was used. Statistical significance is indicated as * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, compared to CTL, and ## p ≤ 0.01, ### p ≤ 0.001 compared to PROK1 or PROK2 treatments when used alone.